Biological Macromolecules Charaterization

  • Aide
  • Recherche
  • RSS
  • Google +
  • Facebook
  • Twitter
Université de Pau et des Pays de l'Adour (UPPA)

"UPPA Connect"

"UPPA Connect" : renforcer les liens entreprises-étudiants

L’UPPA lance sa plate-forme "UPPA Connect" permettant de créer un réseau professionnel entre les entreprises du territoire, les étudiants et les anciens étudiants devenus des professionnels. En savoir plus

Actualités

    • UE Libres

      Inscriptions aux semestres impairs aux UE libres à partir du mardi 12 septembre 2017 (7h)

      Lire la suite

Témoignages
Voir les témoignages de nos étudiants

 

 

Biological Macromolecules Charaterization

Présentation

Introduction into proteomics and the related modern analytical methods (including proteomic mass spectrometry) for proteome analysis of biological organisms.

The following topics are covered:

  • Peptides and proteins; their structure and functions; post-translational modifications
  • The proteomic workflow
  • Sample preparation (fractionation, centrifugation, extraction, interferences, denaturation, reduction, alkylation, enzymatic digestion etc.)
  • Peptide and protein separation methods, gel electrophoresis
  • Proteomic mass spectrometry (ESI and MALDI, high resolution mass analyzers, tandem mass spectrometry, collision induced fragmentation)
  • Data treatment (fingerprint, peptide and protein sequencing, de novo sequencing)
  • Data bases
  • Bottom-up and top-down approaches
  • Exercises: analyses of spectra, peptide sequencing

Analysis, presentation and discussion of scientific articles

Objectifs

Interdisciplinary qualification is intended in chemistry and biology (with respect to proteomics):

  • Biochemical knowledge on peptides and proteins, knowledge in analytical chemistry on modern proteomic methods
  • Interpretation of mass spectra of peptides and proteins; peptide sequencing
  • Interpretation, presentation, discussion and criticism of scientific articles

Volume horaire

  • CM : 10.5h
  • TD : 9h

Examens

Contrôle continu 50% ; Examen terminal 50%

En bref

Crédits ECTS 2

Nombre d'heures 19.5h

Niveau d'étude BAC +5

Type d'enseignement

  • Formation initiale

Langue d'enseignement
English

Contact(s)

Composante

Lieu(x)

  • Pau