Biological Macromolecules Characterization

Présentation

Introduction into proteomics and the related modern analytical methods (including proteomic mass spectrometry) for proteome analysis of biological organisms.

The following topics are covered:

  • Peptides and proteins; their structure and functions; post-translational modifications
  • The proteomic workflow
  • Sample preparation (fractionation, centrifugation, extraction, interferences, denaturation, reduction, alkylation, enzymatic digestion etc.)
  • Peptide and protein separation methods, gel electrophoresis
  • Proteomic mass spectrometry (ESI and MALDI, high resolution mass analyzers, tandem mass spectrometry, collision induced fragmentation)
  • Data treatment (fingerprint, peptide and protein sequencing, de novo sequencing)
  • Data bases
  • Bottom-up and top-down approaches
  • Exercises: analyses of spectra, peptide sequencing

Analysis, presentation and discussion of scientific articles

Objectifs

Interdisciplinary qualification is intended in chemistry and biology (with respect to proteomics):

  • Biochemical knowledge on peptides and proteins, knowledge in analytical chemistry on modern proteomic methods
  • Interpretation of mass spectra of peptides and proteins; peptide sequencing
  • Interpretation, presentation, discussion and criticism of scientific articles

Volume horaire

  • Cours Magistral : 9h
  • Travaux Dirigés : 9h

Examens

Contrôle continu 50% ; Examen terminal 50%

En bref

Crédits ECTS 2.0

Nombre d'heures 18.0

Niveau d'étude BAC +5

Type d'enseignement

  • Formation initiale

Contact(s)

Composante

Lieu(x)

  • Pau