Introduction into proteomics and the related modern analytical methods (including proteomic mass spectrometry) for proteome analysis of biological organisms. Brief introduction into DNA sequencing, genomics and the related analytical methods. Hot topics will be integrated such as corona virus sequencing and Covid-19 tests.

The following topics are covered:

• Peptides and proteins; their structure and functions; post-translational modifications
• DNA sequencing and genomics
• The proteomic workflow
• Sample preparation (fractionation, centrifugation, extraction, interferences, denaturation, reduction, alkylation, enzymatic digestion etc.)
• Peptide and protein separation methods, gel electrophoresis
• Proteomic mass spectrometry (ESI and MALDI, high resolution mass analyzers, tandem mass spectrometry, collision induced fragmentation)
• Data treatment (fingerprint, peptide and protein sequencing, de novo sequencing)
• Data bases
• Bottom-up and top-down approaches
• Exercises: analyses of spectra, peptide sequencing

Analysis, presentation and discussion of scientific articles

At the end of this course you will be able to :

• Biochemical knowledge on peptides and proteins, knowledge in analytical chemistry on modern proteomic methods, basic knowledge on DNA sequencing
• Interpretation of mass spectra of peptides and proteins; peptide sequencing
• Interpretation, presentation, discussion and criticism of scientific articles

1st: continuous assessment :

• 50% final exam : analyze an article
• 50% oral presentation

2nd : written exam about the lesson